MAB4764

antibody from Novus Biologicals

Targeting: AZGP1 ZA2G, ZAG

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB4764

Provider

Novus Biologicals

Product name

Mouse Monoclonal ZAG Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human ZAG in ELISAs.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human ZAG by Simple Western^TM. Simple Western lane view shows lysates of human prostate tissue, loaded at 0.2 mg/mL. A specific band was detected for ZAG at approximately 59 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human ZAG Monoclonal Antibody (Catalog # MAB4764). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human ZAG by Western Blot. Western blot shows human plasma and lysates of human prostate tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human ZAG Monoclonal Antibody (Catalog # MAB4764) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ZAG at approximately 41 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.