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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [3]
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- Product number
- TA501255 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA501255, RRID:AB_11125097
- Product name
- TACC3 mouse monoclonal antibody, clone OTI8B7 (formerly 8B7)
- Antibody type
- Monoclonal
- Description
- TACC3 mouse monoclonal antibody, clone OTI8B7 (formerly 8B7)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- TACC3
- Isotype
- IgG
- Antibody clone number
- OTI8B7
- Vial size
- 100 µl
- Concentration
- 0.62 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TACC3 (RC210754, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TACC3.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-TACC3 monoclonal antibody.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-TACC3 mouse monoclonal antibody (TA501255) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TACC3(RC210754).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-TACC3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501255)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-TACC3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501255)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Hela cells, using anti-TACC3 antibody(TA501255),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC210754 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-TACC3 antibody(TA501255), and then analyzed by flow cytometry.
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Jurkat cells, using anti-TACC3 antibody(TA501255),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
- Validation comment
- FC
