TA501263

antibody from OriGene

Targeting: TACC3 ERIC-1, ERIC1, maskin, Tacc4

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA501263

Provider

OriGene

Proper citation

OriGene Cat#TA501263, RRID:AB_11124173

Product name

TACC3 mouse monoclonal antibody, clone OTI1E11 (formerly 1E11)

Antibody type

Monoclonal

Description

TACC3 mouse monoclonal antibody, clone OTI1E11 (formerly 1E11)

Host

Mouse

Conjugate

Unconjugated

Epitope

TACC3

Isotype

IgG

Antibody clone number

OTI1E11

Vial size

100 µl

Concentration

0.67 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TACC3 (RC210754, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TACC3.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-TACC3 monoclonal antibody.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-TACC3 mouse monoclonal antibody (TA501263) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TACC3(RC210754).

Validation comment

IF

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either RC210754 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-TACC3 antibody(TA501263), and then analyzed by flow cytometry.

Validation comment

FC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Flow cytometric Analysis of Jurkat cells, using anti-TACC3 antibody(TA501263),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).

Validation comment

FC