Antibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Western blot [2]
- Flow cytometry [3]
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Validation data
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- Product number
- NBP2-27332 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal TLR7 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references EV71 infection induces neurodegeneration via activating TLR7 signaling and IL-6 production.
Interferon inducible X-linked gene CXorf21 may contribute to sexual dimorphism in Systemic Lupus Erythematosus.
The role of platelets in mediating a response to human influenza infection.
Deprived TLR9 expression in apparently healthy nasal mucosa might trigger polyp-growth in chronic rhinosinusitis patients.
Distinct autoantibody profiles in systemic lupus erythematosus patients are selectively associated with TLR7 and TLR9 upregulation.
Differential regulation of TLR mediated innate immune response of mouse neuronal cells following infection with novel ECSA genotype of Chikungunya virus with and without E1:A226V mutation.
An unconventional role for miRNA: let-7 activates Toll-like receptor 7 and causes neurodegeneration.
Luo Z, Su R, Wang W, Liang Y, Zeng X, Shereen MA, Bashir N, Zhang Q, Zhao L, Wu K, Liu Y, Wu J
PLoS pathogens 2019 Nov;15(11):e1008142
PLoS pathogens 2019 Nov;15(11):e1008142
Interferon inducible X-linked gene CXorf21 may contribute to sexual dimorphism in Systemic Lupus Erythematosus.
Odhams CA, Roberts AL, Vester SK, Duarte CST, Beales CT, Clarke AJ, Lindinger S, Daffern SJ, Zito A, Chen L, Jones LL, Boteva L, Morris DL, Small KS, Fernando MMA, Cunninghame Graham DS, Vyse TJ
Nature communications 2019 May 15;10(1):2164
Nature communications 2019 May 15;10(1):2164
The role of platelets in mediating a response to human influenza infection.
Koupenova M, Corkrey HA, Vitseva O, Manni G, Pang CJ, Clancy L, Yao C, Rade J, Levy D, Wang JP, Finberg RW, Kurt-Jones EA, Freedman JE
Nature communications 2019 Apr 16;10(1):1780
Nature communications 2019 Apr 16;10(1):1780
Deprived TLR9 expression in apparently healthy nasal mucosa might trigger polyp-growth in chronic rhinosinusitis patients.
Tengroth L, Arebro J, Kumlien Georén S, Winqvist O, Cardell LO
PloS one 2014;9(8):e105618
PloS one 2014;9(8):e105618
Distinct autoantibody profiles in systemic lupus erythematosus patients are selectively associated with TLR7 and TLR9 upregulation.
Chauhan SK, Singh VV, Rai R, Rai M, Rai G
Journal of clinical immunology 2013 Jul;33(5):954-64
Journal of clinical immunology 2013 Jul;33(5):954-64
Differential regulation of TLR mediated innate immune response of mouse neuronal cells following infection with novel ECSA genotype of Chikungunya virus with and without E1:A226V mutation.
Priya R, Dhanwani R, Patro IK, Rao PV, Parida MM
Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases 2013 Dec;20:396-406
Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases 2013 Dec;20:396-406
An unconventional role for miRNA: let-7 activates Toll-like receptor 7 and causes neurodegeneration.
Lehmann SM, Krüger C, Park B, Derkow K, Rosenberger K, Baumgart J, Trimbuch T, Eom G, Hinz M, Kaul D, Habbel P, Kälin R, Franzoni E, Rybak A, Nguyen D, Veh R, Ninnemann O, Peters O, Nitsch R, Heppner FL, Golenbock D, Schott E, Ploegh HL, Wulczyn FG, Lehnardt S
Nature neuroscience 2012 Jun;15(6):827-35
Nature neuroscience 2012 Jun;15(6):827-35
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TLR7 Antibody (4G6) [NBP2-27332] - TLR7 antibodies tested at 2 ug/ml on recombinant partial hTLR7 protein (amino acids 562-839). NB100-56682 and NBP2-24767 immunogen sequences are not present in recombinant protein; *NB100-56588 is not recommended for use in western blot.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TLR7 Antibody (4G6) [NBP2-27332] - Analysis using Azide Free version of NBP2-27332. Human TLR7 antibody in Ramos and transfected 293T lysate using TLR7 monoclonal antibody at 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: TLR7 Antibody (4G6) [NBP2-27332] - Analysis of TLR7 in human monocytes using 2 ug of TLR7 monoclonal antibody (red) and 2 ug of mouse IgG1 isotype control antibody (green). TLR intracellular flow kit was used for this test, and an anti-mouse IgG PE conjugated secondary antibody .
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: TLR7 Antibody (4G6) [NBP2-27332] - Analysis using Azide Free version of NBP2-27332. THP-1 cells were stained with TLR7 (4G) NBP2-25274 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow (Intracellular): TLR7 Antibody (4G6) [NBP2-27332] - Analysis using the PE conjugate of NBP2-27332. Staining of TLR7 in 10^6 human BDCM cells (red) and 0.5 ug of mouse IgG1 isotype control (green). this antibody was used for this test.