PA1-28109

antibody from Invitrogen Antibodies

Targeting: TLR7

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-28109

Provider

Invitrogen Antibodies

Product name

TLR7 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. This antibody will cross-react with mouse TLR7. In human Ramos cells, additional bands are seen.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

50 µg

Concentration

1.0 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

TLR7 in B cells promotes renal inflammation and Gd-IgA1 synthesis in IgA nephropathy.

Zheng N, Xie K, Ye H, Dong Y, Wang B, Luo N, Fan J, Tan J, Chen W, Yu X
JCI insight 2020 Jul 23;5(14)

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of TLR7 in TLR7 using a TLR7 polyclonal antibody (Product # PA1-28109) at a dilution of 3 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of TLR7 was performed by loading RAW264.7 cell lysate. Proteins were transferred to a membrane and probed with a TLR7 Polyclonal Antibody (Product # PA1-28109).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of TLR7 in Ramos cells. Cells were fixed with 10% formalin, permeabilized with 0.5% Triton-X100 and TLR7 Polyclonal Antibody (Product # PA1-28109) (Green) at a dilution of 1:50 was used as a primary antibody. Red : Tubulin. Blue: DAPI.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of TLR7 in RAW264.7 cells using TLR7 Polyclonal Antibody (Product # PA1-28109) (Green) at a dilution of 1:10. Red : Tubulin. Blue: DAPI.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of TLR7 in human colon tissue using TLR7 Polyclonal Antibody (Product # PA1-28109) at a dilution of 5 µg/mL. TOP : primary antibodyBottom : isotype control

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometry analysis of PEX19 was performed in mouse splenocytes using PEX19 Monoclonal Antibody (GT533) (Product # MA5-17281) (Red). Green : isotype control.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis analysis of human peripheral blood mononuclear cell (PBMC) using TLR7 Polyclonal Antibody (Product # PA1-28109). Red: Primary antibody. Green: isotype control. Shaded histogram: cell only. Dilution: 0.5 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometry analysis of ATP6IP2 was performed in human monocyte using a ATP6IP2 Polyclonal Antibody (Product # PA1-32079) (Red) at a dilution of 0.5 µg/10^6 cells. Green : isotype control. Shaded histogram : cells only.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometry analysis of GADD34 was performed in human peripheral blood mononuclear cells (PBMCs) using GADD34 Polyclonal Antibody (Product # PA1-33115) (Red). Green : isotype control.