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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- MAB7156 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rat Monoclonal TLR7 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse TLR7 in direct ELISAs and mouse and rat TLR7 in Western blots. In direct ELISAs, no cross-reactivity with recombinant human TLR7 or recombinant mouse TLR8 is observed.
- Reactivity
- Mouse, Rat
- Host
- Rat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Ligation of TLR7 on CD19(+) CD1d(hi) B cells suppresses allergic lung inflammation via regulatory T cells.
Khan AR, Amu S, Saunders SP, Hams E, Blackshields G, Leonard MO, Weaver CT, Sparwasser T, Sheils O, Fallon PG
European journal of immunology 2015 Jun;45(6):1842-54
European journal of immunology 2015 Jun;45(6):1842-54
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Detection of Mouse and Rat TLR7 by Western Blot. Western blot shows lysates of mouse thymus tissue and NR8383 rat alveolar macrophage cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse/Rat TLR7 Monoclonal Antibody (Catalog # MAB7156) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody. A specific band was detected for TLR7 at approximately 130 to 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
