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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
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- Product number
- MAB1062 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB1062, RRID:AB_1581385
- Product name
- TLR7 monoclonal antibody, clone 4F4
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against partial recombinant TLR7.
- Isotype
- IgG
- Antibody clone number
- 4F4
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references TLR7: A new sensor of viral infection.
Molecular basis for the immunostimulatory activity of guanine nucleoside analogs: activation of Toll-like receptor 7.
The adaptor molecule TIRAP provides signalling specificity for Toll-like receptors.
Crozat K, Beutler B
Proceedings of the National Academy of Sciences of the United States of America 2004 May 4;101(18):6835-6
Proceedings of the National Academy of Sciences of the United States of America 2004 May 4;101(18):6835-6
Molecular basis for the immunostimulatory activity of guanine nucleoside analogs: activation of Toll-like receptor 7.
Lee J, Chuang TH, Redecke V, She L, Pitha PM, Carson DA, Raz E, Cottam HB
Proceedings of the National Academy of Sciences of the United States of America 2003 May 27;100(11):6646-51
Proceedings of the National Academy of Sciences of the United States of America 2003 May 27;100(11):6646-51
The adaptor molecule TIRAP provides signalling specificity for Toll-like receptors.
Horng T, Barton GM, Flavell RA, Medzhitov R
Nature 2002 Nov 21;420(6913):329-33
Nature 2002 Nov 21;420(6913):329-33
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Supportive validation
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- Abnova Corporation (provider)
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- Experimental details
- Western blot analysis of human Ramos cell lysate (80 ug) were resolved by SDS - PAGE , transferred to PVDF membrane and probed with TLR7 monoclonal antibody , clone 4F4 (1 : 1000) (Cat # MAB1062). Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and an ECL detection system. Arrow indicates TLR7 protein in human Ramos cells.
