Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-11605 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TLR7 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Imiquimod Targets Toxoplasmosis Through Modulating Host Toll-Like Receptor-MyD88 Signaling.
Hamie M, Najm R, Deleuze-Masquefa C, Bonnet PA, Dubremetz JF, El Sabban M, El Hajj H
Frontiers in immunology 2021;12:629917
Frontiers in immunology 2021;12:629917
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TLR7 using a TLR7 polyclonal antibody (Product # PA5-11605) in mouse liver tissue lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis using a TLR7 polyclonal antibody (Product # PA5-11605) in Ramos cell lysates (35 µg per lane).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of formalin-fixed, paraffin-embedded human cancer tissue using a TLR7 polyclonal antibody (Product # PA5-11605), followed by HRP-conjugated secondary antibody and AEC staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 Imiquimod increases the expression levels of TLR-7,-11, and TLR-12. (A) Timeline schedule for assessment of TLR(s) profile in BALB/c mice. Briefly, BALB/c mice were injected with 250 tachyzoites/mouse of 76K. After treatment with 50 mug/mouse of Imiquimod or Sulfadiazine (200 mg/L in drinking water), mice were sacrificed at days 35, 42, 49, respectively. Quantitative Real-Time PCR for TLR-11, TLR-12, and TLR-7 (five mice per condition) from brains of these mice. TLRs expression was normalized to GAPDH. The results are expressed as percentage of untreated control (+-) SD. The Anova one-way test was performed to validate significance. *, **, and *** indicate p -values