Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Flow cytometry [4]
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- Product number
- PA5-23488 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TLR7 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Suggested positive control: antigen standard for TLR7 (transient overexpression lysate), Ramos.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of TLR7 in formalin-fixed paraffin-embedded human brain. Samples were incubated in TLR7 polyclonal antibody (Product # PA5-23488) using a dilution of 0.0555555555555556. Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10 mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Neuronal staining of TLR7 was observed. Staining was performed by Histowiz.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Intracellular flow analysis of TLR7 in 10^6 ThP1 cells using 2 µg of TLR7 polyclonal antibody (Product # PA5-23488). Shaded histogram represents cells without antibody; green represents isotype control; red represents TLR7 polyclonal antibody (Product # PA5-23488). An anti-rabbit IgG PE conjugated secondary.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of TLR7 in 10^6 ThP1 cells. Samples were incubated in TLR7 polyclonal antibody (Product # PA5-23488) using a dilution of 2 µg followed by anti-rabbit IgG PE conjugated secondary. Shaded histogram represents cells without antibody. Green: isotype control. Red: TLR7 antibody. Intracellular flow kit was used for this test.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of TLR7 in PBMCs (monocytes). Samples were incubated in TLR7 polyclonal antibody (Product # PA5-23488) using a dilution of 0.5 µg/10^6 cells. The shaded histogram represents cells alone. Green: PE-conjugated rabbit IgG isotype control. Red: TLR7 antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of TLR7 in TLR7/HEK 293 cells. Samples were incubated in TLR7 polyclonal antibody (Product # PA5-23488) using a dilution of 2 µg/10^6 cells. Green: Vector/HEK 293 cells. Red: TLR7/HEK 293 cells. Antibody conjugated to FITC.