AF6796

antibody from Novus Biologicals

Targeting: TMSB4X TB4X, TMSB4

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Antibody Data

Product number

AF6796

Provider

Novus Biologicals

Product name

Sheep Polyclonal Thymosin beta 4 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human Thymosin beta 4 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) Thymosin beta 10 and rhThymosin beta 16 is observed.

Reactivity

Human

Host

Sheep

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human Thymosin beta 4 by Western Blot. Western blot shows lysates of human peripheral blood lymphocytes (PBL), HL-60 human acute promyelocytic leukemia cell line, and human mature dendritic cells. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human Thymosin beta 4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6796) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Thymosin beta 4 at approximately 5 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.