PA5-47875

antibody from Invitrogen Antibodies

Targeting: TMSB4X TB4X, TMSB4

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-47875

Provider

Invitrogen Antibodies

Product name

TMSB4X Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) Thymosin beta 10 and rhThymosin beta 16 is observed.

Concentration

0.2 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis from lysates of human peripheral blood lymphocytes (PBL), HL-60 human acute promyelocytic leukemia cell line, and human mature dendritic cells. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-human Thymosin ß4 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47875) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody. A specific band was detected for Thymosin ß4 at approximately 5 kDa (as indicated). This experiment was conducted under reducing conditions.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of TMSB4X in human peripheral blood lymphocytes (PBL), HL‚60 human acute promyelocytic leukemia cell line, and human mature dendritic cells. Samples were incubated in TMSB4X polyclonal antibody (Product # PA5-47875) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Sheep IgG secondary antibody. A specific band was detected for IRF2BP1 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of TMSB4X in 0.2 mg/mL lysates of HL-60 human acute promyelocytic leukemia cell line. Samples were incubated in TMSB4X polyclonal antibody (Product # PA5-47875) using a dilution of 10 µg/mL followed by HRP-conjugated Anti-Sheep IgG at a dilution of 0.0763888888888889. A specific band was detected for Thymosin β4 at approximately 8 kDa (as indicated). This experiment was conducted under reducing conditions and using the 2-40 kDa separation system.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of TMSB4X in immersion fixed HeLa human cervical epithelial carcinoma cell line. Samples were incubated in TMSB4X polyclonal antibody (Product # PA5-47875) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cytoplasm.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of Thymosin ß4 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-human Thymosin ß4 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47875) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper pane and counterstained with DAPI (blue, lower panel). Specific staining was localized to cytoplasm.