NB600-1443

antibody from Novus Biologicals

Targeting: IL12A CLMF, IL-12A, NFSK, NKSF1, p35

Western blot (Data presented on provider website)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB600-1443

Provider

Novus Biologicals

Proper citation

Novus Cat#NB600-1443, RRID:AB_2295838

Product name

Goat Polyclonal IL-12 Antibody

Antibody type

Polyclonal

Description

Protein G purified. Based on ELISA, the antibody shows ~10% cross-reactivity with recombinant human IL-12 and

Reactivity

Mouse

Host

Goat

Isotype

IgG

Vial size

0.1 mg

Concentration

1.0 mg/ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Radiation Inhibits Interleukin-12 Production via Inhibition of C-Rel through the Interleukin-6/ Signal Transducer and Activator of Transcription 3 Signaling Pathway in Dendritic Cells.

Lee EJ, Lee SJ, Kim JH, Kim KJ, Yang SH, Jeong KY, Seong J
PloS one 2016;11(1):e0146463

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Zwitterionic hydrogels implanted in mice resist the foreign-body reaction.

Zhang L, Cao Z, Bai T, Carr L, Ella-Menye JR, Irvin C, Ratner BD, Jiang S
Nature biotechnology 2013 Jun;31(6):553-6

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: IL-12 Antibody [NB600-1443] - Irradiation-induced IL-6 suppressed IL-12 expression in tumors of hepatoma-bearing mice in vivo and DCs in vitro.IL-6 and IL-12 were measured at 1, 3, and 7 days after 10 Gy irradiation of tumors. Shown are the expressions of IL-6 and IL-12 in MIH-2 tumors (* P < 0.05). BMDCs were prepared from bone marrow of mice and stimulated with LPS. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0146463), licensed under a CC-BY licence.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: IL-12 Antibody [NB600-1443] - Irradiation inhibited IL-12 expression in tumors of hepatoma-bearing mice in vivo and DCs in vitro. HCa-1 and MIH-2 cells were injected intramuscularly into the right thighs of the mice, and tumors were irradiated with 10 Gy of radiation. Shown are IL-12 and DC (CD11c+) expressions at 1, 3, and 7 days after irradiation in HCa-1 tumors in the spleens of HCa-1 bearing mice (** P < 0.01). Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0146463), licensed under a CC-BY licence.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: IL-12 Antibody [NB600-1443] - Irradiation inhibited IL-12 expression in tumors of hepatoma-bearing mice in vivo and DCs in vitro. HCa-1 and MIH-2 cells were injected intramuscularly into the right thighs of the mice, and tumors were irradiated with 10 Gy of radiation. Shown are IL-12 and DC (CD11c+) expressions at 1, 3, and 7 days after irradiation in MIH-2 tumors in the spleens of HCa-1 bearing mice (** P < 0.01). Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0146463), licensed under a CC-BY licence.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: IL-12 Antibody [NB600-1443] - Irradiation-induced IL-6 suppressed IL-12 expression in tumors of hepatoma-bearing mice in vivo and DCs in vitro. IL-6 and IL-12 were measured at 1, 3, and 7 days after 10 Gy irradiation of tumors. Shown are the expressions of IL-6 and IL-12 in HCa-1 tumors (* P < 0.05). BMDCs were prepared from bone marrow of mice and stimulated with LPS. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0146463), licensed under a CC-BY licence.