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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- NBP1-78112 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-78112, RRID:AB_11040416
- Product name
- Rabbit Polyclonal IL-12 p70/IL-12A Antibody
- Antibody type
- Polyclonal
- Description
- Protein A purified. This antibody is specific for Borrelia burgdorferi OspA protein
- Reactivity
- Bacteria
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- LYOPH
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: IL-12 p70/IL-12A Antibody [NBP1-78112] - OspA fusion protein (arrow; expected MW: 70.5 kDa). Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 um nitrocellulose. After blocking with 1% BSA-TTBS (diluted to 1X) overnight at 4 degrees C, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit secondary antibody was used at 1:40,000 in blocking buffer and imaged on the imaging system
