Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- AF912 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal IL-12 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects porcine IL-12 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse IL-12 is observed.
- Reactivity
- Porcine
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Cytokine responses in gnotobiotic pigs after infection with virulent or attenuated human rotavirus.
Azevedo MS, Yuan L, Pouly S, Gonzales AM, Jeong KI, Nguyen TV, Saif LJ
Journal of virology 2006 Jan;80(1):372-82
Journal of virology 2006 Jan;80(1):372-82
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Cell Proliferation Induced by IL-12 and Neutralization by Porcine IL-12 Antibody. Recombinant Porcine IL-12 (Catalog # 912-PL) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine IL-12 (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Porcine IL-12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF912). The ND50 is typically 0.01-0.04 µg/mL.