Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- MAB219-100 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal IL-12 p70/IL-12A Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human IL-12 p70 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) IL-12 p40 homodimer or rhIL-23 is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- IL-12 p70 in Human Kidney. IL-12 p70 in Human Kidney.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by IL-12 and Neutralization by Human IL-12 p70 Antibody. Recombinant Human IL-12 (Catalog # 219-IL) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-12 (1 ng/mL) is neutralized (green line) by increasing concentrations of Human IL-12 p70 Monoclonal Antibody (Catalog # MAB219). The ND50 is typically 0.3-0.9 µg/mL.