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- Antibody Data
- Antigen structure
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- ELISA [1]
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- Product number
- GTX79487 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX79487, RRID:AB_11162473
- Product name
- IL12 antibody [20C2]
- Antibody type
- Monoclonal
- Reactivity
- Human, Bacteria
- Host
- Rat
- Storage
- -20¢XC
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- Sandwich ELISA analysis of human IL12 protein in duplicate at 600, 240, 96, 38.4, 15.4, 0 pg/ml (50 ul per lane) using GTX79487 as capture antibody (7 μg/ml) and GTX79488 as detection antibody (0.03125 μg/ml). The plate was washed then incubated with Streptavidin-HRP. Detection was performed with TMB substrate. The plate was then stopped with 0.16M sulfuric acid. Absorbances were read on a spectrophotometer at 450-550 nm.
