MAB611-100

antibody from R&D Systems

Targeting: IL12A CLMF, IL-12A, NFSK, NKSF1, p35

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB611-100

Provider

R&D Systems

Product name

Human IL-12 p70 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human IL-12 p70 in ELISAs. In sandwich immunoassays, no cross-reactivity with recombinant human (rh) IL-12 p40, rhIL-12 p35, rhIL-23 or recombinant mouse IL-12 is observed.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

P29459 (p35) & P29460 (p40)


Isotype

IgG

Antibody clone number

24945

Vial size

100 ug

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Histamine Regulates Actin Cytoskeleton in Human Toll-like Receptor 4-activated Monocyte-derived Dendritic Cells Tuning CD4+ T Lymphocyte Response.

Aldinucci A, Bonechi E, Manuelli C, Nosi D, Masini E, Passani MB, Ballerini C
The Journal of biological chemistry 2016 Jul 8;291(28):14803-14

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TLR3 and Rig-like receptor on myeloid dendritic cells and Rig-like receptor on human NK cells are both mandatory for production of IFN-gamma in response to double-stranded RNA.

Perrot I, Deauvieau F, Massacrier C, Hughes N, Garrone P, Durand I, Demaria O, Viaud N, Gauthier L, Blery M, Bonnefoy-Berard N, Morel Y, Tschopp J, Alexopoulou L, Trinchieri G, Paturel C, Caux C
Journal of immunology (Baltimore, Md. : 1950) 2010 Aug 15;185(4):2080-8

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Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo.

Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB
AIDS research and human retroviruses 2008 Mar;24(3):453-62

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Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.

Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM
Blood 2007 May 15;109(10):4272-9

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HIV-1 pathogenesis differs in rectosigmoid and tonsillar tissues infected ex vivo with CCR5- and CXCR4-tropic HIV-1.

Grivel JC, Elliott J, Lisco A, Biancotto A, Condack C, Shattock RJ, McGowan I, Margolis L, Anton P
AIDS (London, England) 2007 Jun 19;21(10):1263-72

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Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines.

Copeland S, Siddiqui J, Remick D
Journal of immunological methods 2004 Jan;284(1-2):99-106

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Clinical improvement in chronic plaque-type psoriasis lesions after narrow-band UVB therapy is accompanied by a decrease in the expression of IFN-gamma inducers -- IL-12, IL-18 and IL-23.

Piskin G, Tursen U, Sylva-Steenland RM, Bos JD, Teunissen MB
Experimental dermatology 2004 Dec;13(12):764-72

Immunocytochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

IL-12 p70 in Human PBMCs. IL-12 p70 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human IL-12 p70 Monoclonal Antibody (Catalog # MAB611) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.