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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- AF912 - Provider product page
- Provider
- R&D Systems
- Product name
- Porcine IL-12 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects porcine IL-12 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse IL-12 is observed.
- Reactivity
- Porcine
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q28938 (p40), Q29053 (p35)- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Cytokine responses in gnotobiotic pigs after infection with virulent or attenuated human rotavirus.
Azevedo MS, Yuan L, Pouly S, Gonzales AM, Jeong KI, Nguyen TV, Saif LJ
Journal of virology 2006 Jan;80(1):372-82
Journal of virology 2006 Jan;80(1):372-82
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Supportive validation
- Submitted by
- R&D Systems (provider)
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- Experimental details
- Cell Proliferation Induced by IL-12 and Neutralization by Porcine IL-12 Antibody. Recombinant Porcine IL-12 (Catalog # 912-PL) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine IL-12 (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Porcine IL-12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF912). The ND50 is typically 0.01-0.04 µg/mL.
