Explore
Validate
Learn
Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF1760 - Provider product page
- Provider
- R&D Systems
- Product name
- Rat IL-12 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects rat IL-12 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse IL-12 and less than 5% cross-reactivity with recombinant human IL-12 is observed.
- Reactivity
- Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
p40: NP_072133; p35: Q9R103- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Semliki Forest virus-mediated gene therapy of the RG2 rat glioma.
Roche FP, Sheahan BJ, O'Mara SM, Atkins GJ
Neuropathology and applied neurobiology 2010 Dec;36(7):648-60
Neuropathology and applied neurobiology 2010 Dec;36(7):648-60
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by IL-12 and Neutralization by Rat IL-12 Antibody. Recombinant Rat IL-12 (Catalog # 1760-RL) stimulates proliferation in mouse splenocytes in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL-12 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat IL-12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1760). The ND50 is typically 0.05-0.25 µg/mL.
