14-7128-82

antibody from Invitrogen Antibodies
Targeting: IL12A CLMF, IL-12A, NFSK, NKSF1, p35
Provider product page for 14-7128-82
ELISA

Antibody data

Product number
14-7128-82 - Provider product page
Provider
Invitrogen Antibodies
Product name
IL-12 p35 Monoclonal Antibody (B-T21 (BT21)), eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: The B-T21 antibody reacts with the p35 subunit of human interleukin-12 (IL-12). IL-12 is a 70 kDa heterodimeric cytokine composed of two covalently linked chains, p40 and p35, and is secreted primarily by activated B cells. For optimal detection of human IL-12 p70 by ELISA, a combination of purified B-T21 antibody for capture and biotinylated C8.6 antibody for detection is recommended. Applications Reported: The B-T21 antibody has been reported for use as capture antibody in an IL-12 p70 sandwich ELISA. Applications Tested: The B-T21 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human IL-12 p70 in combination with the biotinylated C8.6 (13-7129) antibody for detection and recombinant human IL-12 p70 (14-8129) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1 - 4 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/mL - 15 pg/mL should be included in each ELISA plate. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human
Host
Mouse
Isotype
IgG
Antibody clone number
B-T21 (BT21)
Vial size
100 µg
Concentration
0.5 mg/mL
Storage
4° C
IL12A protein structure - 14-7128-82 shown in red.