Explore
Validate
Learn
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [9]
- Comments [0]
- Validations
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- BAF219 - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-12 Biotinylated Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human IL-12 in ELISAs and Western blots. When used in combination with the monoclonal capture antibody anti-human IL-12 p40 (Catalog # MAB609) in sandwich ELISAs, less than 2.5% cross-reactivity was observed with rhIL-12 p70. When used in combination with the monoclonal capture antibody anti-human IL-12 p70 (Catalog # MAB611) in sandwich ELISAs, less than 0.1% cross-reactivity was observed with rhIL-12 p40, rhIL-12 p35 and rmIL-12.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Biotin
- Antigen sequence
P29459 (p35) & P29460 (p40)- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references TLR-stimulated CD34 stem cell-derived human skin-like and monocyte-derived dendritic cells fail to induce Th17 polarization of naive T cells but do stimulate Th1 and Th17 memory responses.
Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo.
Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays.
Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
HIV-1 pathogenesis differs in rectosigmoid and tonsillar tissues infected ex vivo with CCR5- and CXCR4-tropic HIV-1.
Blockade of TLR9 agonist-induced type I interferons promotes inflammatory cytokine IFN-gamma and IL-17 secretion by activated human PBMC.
Human tumor membrane vesicles modified to express glycolipid-anchored IL-12 by protein transfer induce T cell proliferation in vitro: a potential approach for local delivery of cytokines during vaccination.
Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines.
Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines.
Duraisingham SS, Hornig J, Gotch F, Patterson S
Journal of immunology (Baltimore, Md. : 1950) 2009 Aug 15;183(4):2242-51
Journal of immunology (Baltimore, Md. : 1950) 2009 Aug 15;183(4):2242-51
Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo.
Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB
AIDS research and human retroviruses 2008 Mar;24(3):453-62
AIDS research and human retroviruses 2008 Mar;24(3):453-62
Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays.
Pfleger C, Schloot N, ter Veld F
Journal of immunological methods 2008 Jan 1;329(1-2):214-8
Journal of immunological methods 2008 Jan 1;329(1-2):214-8
Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM
Blood 2007 May 15;109(10):4272-9
Blood 2007 May 15;109(10):4272-9
HIV-1 pathogenesis differs in rectosigmoid and tonsillar tissues infected ex vivo with CCR5- and CXCR4-tropic HIV-1.
Grivel JC, Elliott J, Lisco A, Biancotto A, Condack C, Shattock RJ, McGowan I, Margolis L, Anton P
AIDS (London, England) 2007 Jun 19;21(10):1263-72
AIDS (London, England) 2007 Jun 19;21(10):1263-72
Blockade of TLR9 agonist-induced type I interferons promotes inflammatory cytokine IFN-gamma and IL-17 secretion by activated human PBMC.
Meyers JA, Mangini AJ, Nagai T, Roff CF, Sehy D, van Seventer GA, van Seventer JM
Cytokine 2006 Sep;35(5-6):235-46
Cytokine 2006 Sep;35(5-6):235-46
Human tumor membrane vesicles modified to express glycolipid-anchored IL-12 by protein transfer induce T cell proliferation in vitro: a potential approach for local delivery of cytokines during vaccination.
Nagarajan S, Selvaraj P
Vaccine 2006 Mar 20;24(13):2264-74
Vaccine 2006 Mar 20;24(13):2264-74
Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines.
Copeland S, Siddiqui J, Remick D
Journal of immunological methods 2004 Jan;284(1-2):99-106
Journal of immunological methods 2004 Jan;284(1-2):99-106
Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines.
Copeland S, Siddiqui J, Remick D
Journal of immunological methods 2004 Jan;284(1-2):99-106
Journal of immunological methods 2004 Jan;284(1-2):99-106
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IL-12 in Human PBMCs. IL-12 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with calcium ionomycin and PMA using Goat Anti-Human IL-12 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF219) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
