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- References [3]
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- Product number
- IC2191A - Provider product page
- Provider
- R&D Systems
- Product name
- Anti-Human/Mouse IL-12/IL-35 p35 APC-conjugated Monoclonal Antibody (Clone 27537)
- Antibody type
- Monoclonal
- Antigen
- S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑12/IL-35 p35, Arg23-Ser219
- Description
- Protein A or G purified from ascites
- Reactivity
- Human, Mouse
- Host
- Mouse
- Antigen sequence
P29459- Isotype
- IgG
- Vial size
- 100 Tests
Submitted references Regulatory T Cells and Their Derived Cytokine, Interleukin-35, Reduce Pain in Experimental Autoimmune Encephalomyelitis.
Differential regulation of interleukin-12 (IL-12)/IL-23 by Tim-3 drives T(H)17 cell development during hepatitis C virus infection.
Regulatory T cells that recognize a ubiquitous stress-inducible self-antigen are long-lived suppressors of autoimmune arthritis.
Duffy SS, Keating BA, Perera CJ, Lees JG, Tonkin RS, Makker PGS, Carrive P, Butovsky O, Moalem-Taylor G
The Journal of neuroscience : the official journal of the Society for Neuroscience 2019 Mar 20;39(12):2326-2346
The Journal of neuroscience : the official journal of the Society for Neuroscience 2019 Mar 20;39(12):2326-2346
Differential regulation of interleukin-12 (IL-12)/IL-23 by Tim-3 drives T(H)17 cell development during hepatitis C virus infection.
Wang JM, Shi L, Ma CJ, Ji XJ, Ying RS, Wu XY, Wang KS, Li G, Moorman JP, Yao ZQ
Journal of virology 2013 Apr;87(8):4372-83
Journal of virology 2013 Apr;87(8):4372-83
Regulatory T cells that recognize a ubiquitous stress-inducible self-antigen are long-lived suppressors of autoimmune arthritis.
van Herwijnen MJ, Wieten L, van der Zee R, van Kooten PJ, Wagenaar-Hilbers JP, Hoek A, den Braber I, Anderton SM, Singh M, Meiring HD, van Els CA, van Eden W, Broere F
Proceedings of the National Academy of Sciences of the United States of America 2012 Aug 28;109(35):14134-9
Proceedings of the National Academy of Sciences of the United States of America 2012 Aug 28;109(35):14134-9
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of IL-12/IL-35 p35 in Human PBMCs or Mouse Splenocytes by Flow Cytometry. (A) Human peripheral blood mononuclear cell (PBMCs) or (B) mouse splenocytes either untreated (light orange filled histogram) or treated with LPS (dark orange filled histogram) were stained with Mouse Anti-Human/Mouse IL-12/IL-35 p35 APC-conjugated Monoclonal Antibody (Catalog # IC2191A) or isotype control antibody (Catalog # IC002A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
