Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [4]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-96832 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LRG1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: VTLSPKDCQV FRSDHGSSIS CQPPAEIPGY LPADTVHLAV EFFNLTHLPA NLLQGASKLQ ELHLSSNGLE SLSPEFLRPV PQLRVLDLTR NALTGLPPGL FQASATLDTL VLKENQLEVL EVSWLHGLKA LGHLDLSGNR LRKLPPGLLA NFTLLRTLDL GENQLETLPP DLLRGPLQLE RLHLEGNKLQ VLGKDLLLPQ PDLRYLFLNG NKLARVAAGA FQGLRQLDML DLSNNSLASV PEGLWASLGQ PNWDMRDGFD ISGNPWICDQ NLSDLYRWLQ AQKDKMFSQN DTRCAGPEAV KGQTLLAVAK SQ
- Concentration
- 1.56 mg/mL
Submitted references Single Cell Transcriptome Data Analysis Defines the Heterogeneity of Peripheral Nerve Cells in Homeostasis and Regeneration.
Chen B, Banton MC, Singh L, Parkinson DB, Dun XP
Frontiers in cellular neuroscience 2021;15:624826
Frontiers in cellular neuroscience 2021;15:624826
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of LRG1 in extracts of various cell lines using LRG1 Polyclonal Antibody (Product # PA5-96832) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of LRG1 in paraffin-embedded rat lung using LRG1 Polyclonal Antibody (Product # PA5-96832) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of LRG1 in paraffin-embedded human colon using LRG1 Polyclonal Antibody (Product # PA5-96832) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of LRG1 in paraffin-embedded human esophagus using LRG1 Polyclonal Antibody (Product # PA5-96832) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of LRG1 in paraffin-embedded mouse lung using LRG1 Polyclonal Antibody (Product # PA5-96832) at a dilution of 1:200.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5 Identify epineurial and endoneurial endothelial cells in intact mouse sciatic nerve by immunostaining. (A-D) Control slides staining without adding primary antibodies in the blocking buffer. (E-H) Double staining CD31 with Spock2 showing that Spock2 is strongly expressed in ECs of large diameter blood vessels outside the epineurium (indicated by arrows). Arrowheads show no expression. (I-L) Double staining CD31 with Rgcc showing that Rgcc is strongly expressed in ECs of large diameter blood vessels outside the epineurium (indicated by arrows). Arrowheads show no expression. Rgcc is also strongly expressed in axons inside the endoneurium. (M-P) Double staining CD31 with Lrg1 showing that Lrg1 is expressed in ECs of endoneurium (indicated by arrows). Lrg1 is also strongly expressed in axons inside the endoneurium. Scale bars in (A) , (E) , (I) , and (M) 30 mum.
