PA5-118035

antibody from Invitrogen Antibodies

Targeting: LRG1 LRG

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-118035

Provider

Invitrogen Antibodies

Product name

LRG1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of LRG1 in Lane A: 0.5 mg HepG2 Whole Cell Lysate. Samples were treated with 60 μg of immunomagnetic Protein A/G beads, incubated with polyclonal antibody (Product # PA5-118035) with a dilution of 1:100 , followed by Goat Anti-Rabbit IgG (H+L)/HRP using a dilution of 1:10,000. Assay was performed under reducing conditions. Predicted band size: 38 kDa , Observed band size : 38 kDa .

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

LRG1 Immunoprecipitation using: Lane A: 0.5 mg HepG2 Whole Cell Lysate 2 µL with LRG1 Polyclonal Antibody (Product # PA5-118035) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: LRG1 Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) /HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 38 kDa. Observed band size: 38 kDa.