Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- PA5-31046 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LRG1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: human plasma. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of LRG1 using 30 µg of HepG2 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a LRG1 polyclonal antibody (Product # PA5-31046) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of LRG1 was performed by separating 30 µg of human tissue extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a LRG1 Polyclonal Antibody (Product # PA5-31046) at a dilution of 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- LRG1 Polyclonal Antibody detects LRG1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: LRG1 protein stained by LRG1 Polyclonal Antibody (Product # PA5-31046) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human breast cancer, using LRG1 (Product # PA5-31046) antibody at 1:250 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- LRG1 Polyclonal Antibody detects LRG1 protein at cytosol on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. LRG1 Polyclonal Antibody (Product # PA5-31046) dilution: 1:250. Scale bar = 100 µm. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.