PA5-95499

DYRK1A antibody from Invitrogen Antibodies

DYRK, DYRK1, MNBH

Western blot

Supportive data in Antibodypedia

ELISA

Recommended by provider

Immunocytochemistry

Data presented on provider website

Flow cytometry

Supportive data in Antibodypedia

Provider product page for PA5-95499

Antibody Data

Product number

PA5-95499

Provider

Invitrogen Antibodies

Product name

Anti-DYRK1A Polyclonal Antibody

Provider product page

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Reconstitution information: Add 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

500 µg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of DYRK1A in Lane 1: human HeLa whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: rat skeletal muscle tissue lysates, Lane 4: mouse skeletal muscle tissue lysates. Electrophoresis was performed with 5-20% SDS-PAGE gel (70V, Stacking gel; 90V Resolving gel, Time: 2-3 hours), transferred to a nitrocellulose membrane and blocked using 5% Non-fat Milk/TBS (1.5 hrs at room temperature). Samples were incubated with DYRK1A polyclonal antibody (Product # PA5-95499) using a 0.5 µg/mL dilution, followed by a goat anti-rabbit IgG-HRP at a dilution of 1:10,000, and developed with enhanced chemiluminescence (ECL).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of DYRK1A in SiHa cells (blue line), isotype control rabbit IgG (green line) and unlabeled (red line). Samples were blocked with 10% goat serum, incubated with DYRK1A polyclonal antibody (Product # PA5-95499) at a dilution of 1 µg (per 1x10^6 cells), followed by 488 conjugated goat anti-rabbit IgG (30 min at 20°C) using a 5-10 µg (per 1x10^6 cells) dilution.