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LearnHPA022141
antibody from Atlas Antibodies
Targeting: CDK5RAP3
C53, FLJ13660, HSF-27, IC53, LZAP, MST016, OK/SW-cl.114
Western blot
Immunocytochemistry
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- HPA022141 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA022141, RRID:AB_1846426
- Product name
- Anti-CDK5RAP3
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human CDK5RAP3, Gene description: CDK5 regulatory subunit associated protein 3, Alternative Gene Names: C53, FLJ13660, HSF-27, IC53, LZAP, MST016, OK/SW-cl.114, Validated applications: ICC, IHC, WB, Uniprot ID: Q96JB5, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.2 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references C53 Interacting with UFM1-Protein Ligase 1 Regulates Microtubule Nucleation in Response to ER Stress
Loss of LZAP inactivates p53 and regulates sensitivity of cells to DNA damage in a p53-dependent manner
Klebanovych A, Vinopal S, Dráberová E, Sládková V, Sulimenko T, Sulimenko V, Vosecká V, Macůrek L, Legido A, Dráber P
Cells 2022;11(3):555
Cells 2022;11(3):555
Loss of LZAP inactivates p53 and regulates sensitivity of cells to DNA damage in a p53-dependent manner
Wamsley J, Gary C, Biktasova A, Hajek M, Bellinger G, Virk R, Issaeva N, Yarbrough W
Oncogenesis 2017;6(4):e314-e314
Oncogenesis 2017;6(4):e314-e314
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Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-CDK5RAP3 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
- Sample type
- Human
- Protocol
- Protocol
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli & cytosol.
- Sample type
- Human
