HPA022141

antibody from Atlas Antibodies

Targeting: CDK5RAP3 C53, FLJ13660, HSF-27, IC53, LZAP, MST016, OK/SW-cl.114

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA022141

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA022141, RRID:AB_1846426

Product name

Anti-CDK5RAP3

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

LIGKLTSLQLQHLFMILASPRYVDRVTEFLQQKLK
QSQLLALKKELMVQKQQEALEEQAALEPKLDLLLE
KTKELQKLIEADISKRYSGRPVNLMGTSL


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-CDK5RAP3 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Western blot analysis in human cell line MCF-7.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Western blot analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli & cytosol.

Sample type

HUMAN

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human epididymis shows strong cytoplasmic positivity in glandular cells.

Sample type

HUMAN