PA5-116477

antibody from Invitrogen Antibodies

Targeting: CDK5RAP3 C53, FLJ13660, HSF-27, IC53, LZAP, MST016, OK/SW-cl.114

Western blot

Antibody data

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Validation data

Product number: PA5-116477 - Provider product page
Provider: Invitrogen Antibodies
Product name: CDK5RAP3 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant full-length protein
Description: Positive controls: MCF-7 cell lysates, LOVO, PC-3M, SHSY5Y.
Reactivity: Human
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 1 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

CDK5RAP3 protein structure - PA5-116477 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemical analysis of CDK5RAP3 in LOVO cells using a CDK5RAP3 polyclonal antibody (Product #PA5-116477). Formalin fixed cells, as seen in green, were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody ( 1:100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1:100 dilution. The nuclear counter stain is DAPI (blue).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemical analysis of CDK5RAP3 in PC-3M cells using a CDK5RAP3 polyclonal antibody (Product #PA5-116477). Formalin fixed cells, as seen in green, were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody ( 1:100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1:100 dilution. The nuclear counter stain is DAPI (blue).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemical analysis of CDK5RAP3 in SHSY5Y cells using a CDK5RAP3 polyclonal antibody (Product #PA5-116477). Formalin fixed cells, as seen in green, were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody ( 1:100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1:100 dilution. The nuclear counter stain is DAPI (blue).