PAB20084

antibody from Abnova Corporation

Targeting: PAPOLA PAP

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB20084

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB20084, RRID:AB_10966943

Product name

PAPOLA polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant PAPOLA.

Antigen sequence

EGVKLTALNDSSLDLSMDSDNSMSVPSPTSATKTS
PLNSSGSSQGRNSPAPAVTAASVTNIQATEVSVPQ
VNSSESSGGTSSESIPQTATQPAISPPPKPTVSRV
VSSTRLVNPPPRSSGNAATSGNAATKIPTPIVGVK


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of cell lysates with PAPOLA polyclonal antibody (Cat # PAB20084) at 1:250-1:500 dilution.Lane 1 : NIH/3T3Lane 2 : NBT-II

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-251 MG with PAPOLA polyclonal antibody (Cat # PAB20084) at 1-4 ug/mL dilution shows positivity in nuclei but not nucleoli.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human prostate with PAPOLA polyclonal antibody (Cat # PAB20084) shows moderate nuclear positivity in glandular cells at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)