Antibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
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Validation data
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- Product number
- R1601P - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#R1601P, RRID:AB_1007949
- Product name
- anti Thyroglobulin (Tg)
- Antibody type
- Monoclonal
- Antigen
- This protein A purified monoclonal antibody was produced by repeated immunizations with human thyroglobulin protein. Produced by the fusion between BALB/c mouse splenocytes and mouse myeloma SP2/0 cells after immunization with thyroglobulin from human thyroid gland using conventional hybridoma technology.
- Reactivity
- Human, Mouse, Rat, Canine
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2H11
- Vial size
- 0.1 mg
- Concentration
- 1.6 mg/ml (by UV absorbance at 280 nm)
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Supportive validation
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- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Figure 1. Western blot using Immunochemical's Mouse anti-Thyroglobulin antibody (R1601P). Separation was achieved under reducing conditions using a pre-cast 5% Tris-HCl gel from Bio-Rad Laboratories. This antibody recognizes a single 330 kDa band corresponding to human thyroglobulin (left lane 3 µg, right lane 3.0 µg) as confirmed by the position of molecular weight markers (not shown). A 1/400 dilution of Mab anti-Thyroglobulin was used for 2h followed by detection using a 1/5,000 dilution of IRDyeTM800 conjugated Goat-a-Mouse IgG [H&L] and visualization using the Odyssey ¨ Infrared Imaging System developed by LI-COR. Other detection systems will yield similar results. IRDye is a trademark of LI-COR, Inc.