MA5-24621

antibody from Invitrogen Antibodies

Targeting: NSD2 KMT3G, MMSET, WHSC1

Western blot (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-24621

Provider

Invitrogen Antibodies

Product name

NSD2 Monoclonal Antibody (CL1063)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: SANGKTPSCE VNRECSVFLS KAQLSSSLQE GVMQKFNGHD ALPFIPADKL KDLTSRVFNG EPGAHDAKLR FESQEMKGIG TPPNTTPIKN GSPEIKLKIT KTYMNGKPLF ESSICGD Highest antigen sequence identity to the following orthologs: Mouse - 91%, Rat - 91%. Binds to an epitope located within the peptide sequence ALPFIPADKL as determined by overlapping synthetic peptides.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

CL1063

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Activating transcription factor 2 promotes the progression of hepatocellular carcinoma by inducing the activation of the WHSC1-mediated TOP2A/PI3K/AKT axis.

Bao ZM, Yao D, Qian X, Zhang HG, Yang M, Guo YH, Qin L
The Kaohsiung journal of medical sciences 2022 Jul;38(7):662-674

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of NSD2 in U-251 cells using NSD2 Monoclonal Antibody (CL1063) (Product # MA5-24621), showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of NSD2 in human cerebral cortex using NSD2 Monoclonal Antibody (CL1063) (Product # MA5-24621) shows strong nuclear positivity in neurons.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of NSD2 in human colorectal cancer using NSD2 Monoclonal Antibody (CL1063) (Product # MA5-24621) shows strong nuclear positivity in tumor cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of NSD2 in human liver using NSD2 Monoclonal Antibody (CL1063) (Product # MA5-24621) shows no positivity in hepatocytes as expected.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of NSD2 in human rectum using NSD2 Monoclonal Antibody (CL1063) (Product # MA5-24621) shows strong nuclear positivity in glandular cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of NSD2 in human testis using NSD2 Monoclonal Antibody (CL1063) (Product # MA5-24621) shows moderate to strong nuclear positivity in cells in seminiferous ducts.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

3 FIGURE ATF2 silencing reduces expression of WHSC1 in HCC cells. (A) data in the hTFtarget database suggests WHSC1 as a downstream target of ATF2; (B) conservative binding site of ATF2; (C) putative binding site between WHSC1 and ATF2; (D,E) mRNA (D) and protein (E) expression of WHSC1in clinically collected HCC tumor tissues and the paired normal tissues ( n = 50) examined by RT-qPCR and IHC staining (paired t test); (F) mRNA expression of WHSC1 in HepG2 and SNU-398 cells after ATF2 silencing examined by RT-qPCR (two-way ANOVA); (G) protein level of H3K36me2 in HepG2 and SNU-398 cells after ATF2 silencing determined by western blot analysis (two-way ANOVA); (H), distribution of WHSC1 and H3K36me2 in HepG2 cells examined by fluorescence co-localization assay; (I), abundance of WHSC1 promoter fragments enriched by anti-ATF2 determined by the ChIP-qPCR assay (two-way ANOVA); (J), binding relationship between WHSC1 promoter and ATF2 validated by a dual-luciferase reporter gene assay (two-way ANOVA). Data are expressed as the mean +- SD. Repetition = 3. * p < 0.05; ** p < 0.01; *** p < 0.001