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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- MAB6966 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse Serpin A8/Angiotensinogen Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse Serpin A8/Angiotensinogen in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) Serpin A8/Angiotensinogen, rhSerpin A4, recombinant mouse (rm) Serpin A1, A3N, A11, B8, C1, E1, E2, I1, or rmPEDF is observed.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
P11859- Isotype
- IgG
- Antibody clone number
- 754438
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse Serpin A8/Angiotensinogen by Western Blot. Western blot shows lysates of mouse liver tissue. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Mouse Serpin A8/Angiotensinogen Monoclonal Antibody (Catalog # MAB6966) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands were detected for Serpin A8/Angiotensinogen at approximately 52-62 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Serpin A8/Angiotensinogen in Mouse Intestine. Serpin A8/Angiotensinogen was detected in perfusion fixed frozen sections of mouse intestine using Rat Anti-Mouse Serpin A8/Angiotensinogen Monoclonal Antibody (Catalog # MAB6966) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to intestinal epithelial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
