Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- ABIN2616160 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Angiotensinogen (serpin Peptidase Inhibitor, Clade A, Member 8) (AGT) antibody
- Antibody type
- Polyclonal
- Antigen
- Artificially Synthesized polypeptide, Ang1-7 conjugated to OVA.ImmunogenType: Synthetic peptide
- Description
- Affinity purified
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 μg
- Storage
- Store frozen product at or below -20°C. Thawed product may be stored for 2-4 weeks at 4°C. For optimal storage, aliquot to smaller portions and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
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Supportive validation
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- antibodies-online (provider)
- Main image
- Experimental details
- Histone H4 dimethyl Arg3 symmetric pAb tested by Western blot. Western blot performed using Histone H4 dimethyl Arg3 symmetric pAb at a dilution of 1:2,000. Lane 1: Nuclear extract from Raji cells (20 μg). Lane 2: Nuclear extract from HeLa cells (20 μg).
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- antibodies-online (provider)
- Main image
- Experimental details
- Histone H4 dimethyl Arg3 symmetric pAb tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H4 dimethyl Arg3 symmetric pAb for dimethyl-arginine 3 of histone H4. Peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with Histone H4 dimethyl Arg3 symmetric pAb at 1:2,000. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: unmodified Arg3. Lane 2: dimethyl-Arg3 H4 (symmetric). Lane 3: dimethyl-Arg3 H4 (asymmetric). Lane 4: monomethyl-Arg3 H4. Lane 5: unmodified Arg26 H3. Lane 6: monomethyl-Arg26 H3. Lane 7: dimethyl-Arg26 H3 (asymmetric). Lane 8: dimethyl-Arg26 H3 (symmetric). Lane 9: dimethyl-Arg2 H3 (asymmetric). Lane 10: dimethyl-Arg2 (symmetric). Lane 11: dimethyl-Arg8 H3 (asymmetric). Lane 12: dimethyl-Arg8 H3 (symmetric). Lane 13: dimethyl-Arg17 (asymmetric). Lane 14: dimethyl-Arg17 H3 (symmetric).