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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Chromatin Immunoprecipitation [2]
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Validation data
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- Product number
- ABIN2616168 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Angiotensin II (Ang II) antibody (FITC)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide AngII.ImmunogenType: Synthetic peptide
- Description
- Affinity purified
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Green dye
- Isotype
- IgG
- Vial size
- 100 μg
- Storage
- Store frozen product at or below -20°C. Thawed product may be stored for 2-4 weeks at 4°C. For optimal storage, aliquot to smaller portions and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- BRD3 antibody (pAb) tested by Western blot. Detection of BRD3 by Western blot analysis. Lane 1: Whole cell extract of HeLa cells (20 μg). Lane 2: Whole cell extract of NIH-3T3 cells. Both probed with BRD3 antibody (pAb) at a 1:500 dilution.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- BRD3 antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 30 μg of chromatin from human H526 cells and 10 μl of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 16 million sequence tags were mapped to show the expected promoter localization.
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- BRD3 antibody (pAb) tested by ChIP. Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 30 μg of chromatin from human H526 cells and 10 μl BRD3 antibody. ChIP DNA was used in qPCR with the control primer or gene-specific primer as indicated. Data are presented as Binding Events Detected per 1000 Cells using Active Motif's Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.
