AF2745

antibody from R&D Systems

Targeting: LUM LDC, SLRR2D

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF2745

Provider

R&D Systems

Product name

Mouse Lumican Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects mouse Lumican in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human Lumican is observed.

Reactivity

Mouse

Host

Goat

Conjugate

Unconjugated

Antigen sequence

P51885


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Diet-dependent function of the extracellular matrix proteoglycan Lumican in obesity and glucose homeostasis.

Wolff G, Taranko AE, Meln I, Weinmann J, Sijmonsma T, Lerch S, Heide D, Billeter AT, Tews D, Krunic D, Fischer-Posovszky P, Müller-Stich BP, Herzig S, Grimm D, Heikenwälder M, Kao WW, Vegiopoulos A
Molecular metabolism 2019 Jan;19:97-106

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Origin and differentiation trajectories of fibroblastic reticular cells in the splenic white pulp.

Cheng HW, Onder L, Novkovic M, Soneson C, Lütge M, Pikor N, Scandella E, Robinson MD, Miyazaki JI, Tersteegen A, Sorg U, Pfeffer K, Rülicke T, Hehlgans T, Ludewig B
Nature communications 2019 Apr 15;10(1):1739

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Fibromodulin and Biglycan Modulate Periodontium through TGFβ/BMP Signaling.

Wang L, Foster BL, Kram V, Nociti FH Jr, Zerfas PM, Tran AB, Young MF, Somerman MJ
Journal of dental research 2014 Aug;93(8):780-7

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Lumican, an extracellular matrix proteoglycan, is a novel requisite for hepatic fibrosis.

Krishnan A, Li X, Kao WY, Viker K, Butters K, Masuoka H, Knudsen B, Gores G, Charlton M
Laboratory investigation; a journal of technical methods and pathology 2012 Dec;92(12):1712-25

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Modulation of small leucine-rich proteoglycans (SLRPs) expression in the mouse uterus by estradiol and progesterone.

Salgado RM, Favaro RR, Zorn TM
Reproductive biology and endocrinology : RB&E 2011 Feb 4;9:22

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The estrous cycle modulates small leucine-rich proteoglycans expression in mouse uterine tissues.

Salgado RM, Favaro RR, Martin SS, Zorn TM
Anatomical record (Hoboken, N.J. : 2007) 2009 Jan;292(1):138-53

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Lumican in Mouse Intestine. Lumican was detected in perfusion fixed frozen sections of mouse intestine using Goat Anti-Mouse Lumican Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2745) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.