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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- R32130 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Lumican Antibody / LUM
- Antibody type
- Polyclonal
- Antigen
- Amino acids 68-338 of mouse Lumican were used as the immunogen for the Lumican antibody.
- Description
- Antigen affinity
- Reactivity
- Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 µg
- Concentration
- Lyophilized; resuspend with 200 ul for 0.5 mg/ml
- Storage
- After reconstitution, the Lumican antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of mouse 1) testis, 2) thymus and 3) NIH3T3 lysate with Lumican antibody. Expected/observed moleculer weight: ~40/60 kDa (unmodified/glycosylated). (Ref 1)
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE mouse brain with Lumican antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
