Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-47410 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LUM Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs and Western blots, approximately 50% cross-reactivity with recombinant mouse Lumican is observed.
- Concentration
- 0.2 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis from lysates of human colon tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-human Lumican Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47410) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. A specific band was detected for Lumican at approximately 60-85 kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of LUM in human colon tissue. Samples were incubated in LUM polyclonal antibody (Product # PA5-47410) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Goat IgG secondary antibody. A specific band was detected for Lumican at approximately 60-85 kDa (as indicated). This experiment was conducted under reducing conditions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of LUM in immersion fixed paraffin-embedded sections of human kidney. Samples were incubated in LUM polyclonal antibody (Product # PA5-47410) using a dilution of 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue). Specific staining was localized to convoluted tubules.