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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [3]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- MA5-34828 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LUM Recombinant Rabbit Monoclonal Antibody (JE11-45)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: Jurkat, 293T, Human skin tissue, MCF-7, MG-63, PANC-1, rat kidney tissue, human skin tissue.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- JE11-45
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles, store in dark
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Lumican in Lane 1: Jurkat, Lane 2: 293T, Lane 3: Human skin. Samples were incubated with Lumican monoclonal antibody (Product # MA5-34828), at a dilution of 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Lumican in PANC-1 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Lumican monoclonal antibody (Product # MA5-34828), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Lumican in MG-63 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Lumican monoclonal antibody (Product # MA5-34828), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Lumican in MCF-7 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Lumican monoclonal antibody (Product # MA5-34828), followed by DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Lumican in paraffin-embedded rat kidney tissue. Samples were incubated with Lumican monoclonal antibody (Product # MA5-34828), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Lumican in paraffin-embedded human skin tissue. Samples were incubated with Lumican monoclonal antibody (Product # MA5-34828), and followed by hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of Lumican in 293T cells (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Samples were incubated with Lumican monoclonal antibody (Product # MA5-34828) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.
