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ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [4]
- Flow cytometry [1]
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- Product number
- RQ8519 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Lumican Antibody / LUM
- Antibody type
- Polyclonal
- Description
- This highly specific Lumican antibody is suitable for use in Western blot/Immunohistochemistry/Flow cytometry/ELISA applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the Lumican antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human Jurkat, 2) human placenta, 3) human Caco-2, 4) rat liver, 5) mouse liver and 6) mouse NIH 3T3 cell lysate with Lumican antibody. Predicted molecular weight ~38 kDa but may be observed at higher molecular weights due to glycosylation.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human lung cancer tissue with Lumican antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human esophageal squamous carcinoma tissue with Lumican antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human thyroid papillary carcinoma tissue with Lumican antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human mouse kidney tissue with Lumican antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of fixed and permeabilized human SH-SY5Y cells with Lumican antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Lumican antibody.
