AF2846

antibody from R&D Systems

Targeting: LUM LDC, SLRR2D

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF2846

Provider

R&D Systems

Product name

Human Lumican Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects human Lumican in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse Lumican is observed.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Antigen sequence

P51884


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Hypoxia-induced autophagy of stellate cells inhibits expression and secretion of lumican into microenvironment of pancreatic ductal adenocarcinoma.

Li X, Lee Y, Kang Y, Dai B, Perez MR, Pratt M, Koay EJ, Kim M, Brekken RA, Fleming JB
Cell death and differentiation 2019 Jan;26(2):382-393

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Extracellular lumican inhibits pancreatic cancer cell growth and is associated with prolonged survival after surgery.

Li X, Truty MA, Kang Y, Chopin-Laly X, Zhang R, Roife D, Chatterjee D, Lin E, Thomas RM, Wang H, Katz MH, Fleming JB
Clinical cancer research : an official journal of the American Association for Cancer Research 2014 Dec 15;20(24):6529-40

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Resistance of corneal RFUVA–cross-linked collagens and small leucine-rich proteoglycans to degradation by matrix metalloproteinases.

Zhang Y, Mao X, Schwend T, Littlechild S, Conrad GW
Investigative ophthalmology & visual science 2013 Feb 5;54(2):1014-25

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Lumican, an extracellular matrix proteoglycan, is a novel requisite for hepatic fibrosis.

Krishnan A, Li X, Kao WY, Viker K, Butters K, Masuoka H, Knudsen B, Gores G, Charlton M
Laboratory investigation; a journal of technical methods and pathology 2012 Dec;92(12):1712-25

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HMGA2 overexpression-induced ovarian surface epithelial transformation is mediated through regulation of EMT genes.

Wu J, Liu Z, Shao C, Gong Y, Hernando E, Lee P, Narita M, Muller W, Liu J, Wei JJ
Cancer research 2011 Jan 15;71(2):349-59

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Effects of ultraviolet-A and riboflavin on the interaction of collagen and proteoglycans during corneal cross-linking.

Zhang Y, Conrad AH, Conrad GW
The Journal of biological chemistry 2011 Apr 15;286(15):13011-22

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human Lumican by Western Blot. Western blot shows lysates of human colon tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Lumican Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2846) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Lumican at approximately 60-85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Lumican in Human Kidney. Lumican was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human Lumican Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2846) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.