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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Immunohistochemistry [3]
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- Product number
- NBP2-22440 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal HSP60 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified.
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100ug
- Concentration
- LYOPH
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HSP60 Antibody (4B9/89) [NBP2-22440] - Analysis of 50ug of the indicated whole cell lysates and 15ul of PageRuler Prestained Protein Ladder.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: HSP60 Antibody (4B9/89) [NBP2-22440] - Analysis of Heat Shock Protein 60 (HSP60) was performed on HeLa cells. Antigen:antibody complexes were formed by incubating 500ug whole cell lysate with 2ug of HSP60 monoclonal antibody overnight on a rocking platform at 4C. The immune complexes were captured on 50ul Protein A/G Plus Agarose, washed extensively, and eluted with Lane Marker Reducing Sample Buffer. Samples were then resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a HSP60 monoclonal antibody at a dilution of 1:1000 overnight rotating at 4C, washed in TBST, and probed with Clean-Blot IP Detection Reagent (HRP) at a dilution of 1:1000 for at least one hour. Chemiluminescent detection was performed using SuperSignal West Dura.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HSP60 Antibody (4B9/89) [NBP2-22440] - Cancer biopsies of deparaffinized human Breast carcinoma tissue.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HSP60 Antibody (4B9/89) [NBP2-22440] - Normal deparaffinized human Kidney tissue.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HSP60 Antibody (4B9/89) [NBP2-22440] - Normal deparaffinized human Tonsil tissue.
