H00003329-M01

antibody from Abnova Corporation

Targeting: HSPD1 GroEL, HSP60, SPG13

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00003329-M01

Provider

Abnova Corporation

Product name

HSPD1 monoclonal antibody (M01), clone 2A7

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full-length recombinant HSPD1.

Antigen sequence

MLRLPTVFRQMRPVSRVLAPHLTRAYAKDVKFGAD
ARALMLQGVDLLADAVAVTMGPKGRTVIIEQSWGS
PKVTKDGVTVAKSIDLKDKYKNIGAKLVQDVANNT
NEEAGDGTTTATVLARSIAKEGFEKISKGANPVEI
RRGVMLAVDAVIAELKKQSKPVTTPEEIAQVATIS
ANGDKEIGNIISDAMKKVGRKGVITVKDGKTLNDE
LEIIEGMKFDRGYISPYFINTSKGQKCEFQDAYVL
LSEKKISSIQSIVPALEIANAHRKPLVIIAEDVDG
EALSTLVLNRLKVGLQVVAVKAPGFGDNRKNQLKD
MAIATGGAVFGEEGLTLNLEDVQPHDLGKVGEVIV
TKDDAMLLKGKGDKAQIEKRIQEIIEQLDVTTSEY
EKEKLNERLAKLSDGVAVLKVGGTSDVEVNEKKDR
VTDALNATRAAVEEGIVLGGGCALLRCIPALDSLT
PANEDQKIGIEIIKRTLKIPAMTIAKNAGVEGSLI
VEKIMQSSSEVGYDAMAGDFVNMVEKGIIDPTKVV
RTALLDAAGVASLLTTAEVVVTEIPKEEKDPGMGA
MGGMGGGMGGGMF


Isotype

IgG

Antibody clone number

2A7

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of HSPD1 expression in transfected 293T cell line by HSPD1 monoclonal antibody (M01), clone 2A7.Lane 1: HSPD1 transfected lysate (Predicted MW: 61.1 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged HSPD1 is 0.03 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol