Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- MAB1800 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal HSP60 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human, mouse and rat HSP60.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references C9ORF72-ALS/FTD-associated poly(GR) binds Atp5a1 and compromises mitochondrial function in vivo.
MICU1 Confers Protection from MCU-Dependent Manganese Toxicity.
Comparative analysis of the interaction of HSPs in dendritic cells, macrophages, RGM-1 cells infected by Helicobacter pylori.
Heat shock protein 60: an endogenous inducer of dopaminergic cell death in Parkinson disease.
Nitric oxide-induced apoptosis in RAW 264.7 macrophages is mediated by endoplasmic reticulum stress pathway involving ATF6 and CHOP.
Choi SY, Lopez-Gonzalez R, Krishnan G, Phillips HL, Li AN, Seeley WW, Yao WD, Almeida S, Gao FB
Nature neuroscience 2019 Jun;22(6):851-862
Nature neuroscience 2019 Jun;22(6):851-862
MICU1 Confers Protection from MCU-Dependent Manganese Toxicity.
Wettmarshausen J, Goh V, Huang KT, Arduino DM, Tripathi U, Leimpek A, Cheng Y, Pittis AA, Gabaldón T, Mokranjac D, Hajnóczky G, Perocchi F
Cell reports 2018 Nov 6;25(6):1425-1435.e7
Cell reports 2018 Nov 6;25(6):1425-1435.e7
Comparative analysis of the interaction of HSPs in dendritic cells, macrophages, RGM-1 cells infected by Helicobacter pylori.
Yao Y, Wu J, Gu T, Cheng Y, Li G
American journal of translational research 2016;8(10):4184-4194
American journal of translational research 2016;8(10):4184-4194
Heat shock protein 60: an endogenous inducer of dopaminergic cell death in Parkinson disease.
Noelker C, Morel L, Osterloh A, Alvarez-Fischer D, Lescot T, Breloer M, Gold M, Oertel WH, Henze C, Michel PP, Dodel RC, Lu L, Hirsch EC, Hunot S, Hartmann A
Journal of neuroinflammation 2014 May 8;11:86
Journal of neuroinflammation 2014 May 8;11:86
Nitric oxide-induced apoptosis in RAW 264.7 macrophages is mediated by endoplasmic reticulum stress pathway involving ATF6 and CHOP.
Gotoh T, Oyadomari S, Mori K, Mori M
The Journal of biological chemistry 2002 Apr 5;277(14):12343-50
The Journal of biological chemistry 2002 Apr 5;277(14):12343-50
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human/Mouse/Rat HSP60 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, DA3 mouse myeloma cell line, and Nb2-11 rat lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat HSP60 Monoclonal Antibody (Catalog # MAB1800) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for HSP60 at approximately 62 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human HSP60 by Simple WesternTM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, HeLa human cervical epithelial carcinoma cell line, and HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for HSP60 at approximately 60 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Mouse/Rat HSP60 Monoclonal Antibody (Catalog # MAB1800). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.