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- Immunohistochemistry [4]
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- Product number
- GTX110089 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX110089, RRID:AB_1950529
- Product name
- HSP60 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat, Hamster
- Host
- Rabbit
Submitted references Keratinocyte differentiation induces APOBEC3A, 3B, and mitochondrial DNA hypermutation.
Lead (Pb) induced ATM-dependent mitophagy via PINK1/Parkin pathway.
Aqueous extracts of Paeonia suffruticosa modulates mitochondrial proteostasis by reactive oxygen species-induced endoplasmic reticulum stress in pancreatic cancer cells.
miR-30a Remodels Subcutaneous Adipose Tissue Inflammation to Improve Insulin Sensitivity in Obesity.
PE_PGRS3 of Mycobacterium tuberculosis is specifically expressed at low phosphate concentration, and its arginine-rich C-terminal domain mediates adhesion and persistence in host tissues when expressed in Mycobacterium smegmatis.
Mitophagy inhibits proliferation by decreasing cyclooxygenase-2 (COX-2) in arsenic trioxide-treated HepG2 cells.
ATM mediates spermidine-induced mitophagy via PINK1 and Parkin regulation in human fibroblasts.
Cadmium induces mitophagy through ROS-mediated PINK1/Parkin pathway.
Mutations in the SPG7 gene cause chronic progressive external ophthalmoplegia through disordered mitochondrial DNA maintenance.
Proteomic analysis of rhein-induced cyt: ER stress mediates cell death in breast cancer cells.
Hyaluronic acid-dependent protection against UVB-damaged human corneal cells.
Quercetin-induced cardioprotection against doxorubicin cytotoxicity.
Basal and stress-induced Hsp70 are modulated by ataxin-3.
Quantitative proteomic analyses of human cytomegalovirus-induced restructuring of endoplasmic reticulum-mitochondrial contacts at late times of infection.
Wakae K, Nishiyama T, Kondo S, Izuka T, Que L, Chen C, Kase K, Kitamura K, Mohiuddin M, Wang Z, Ahasan MM, Nakamura M, Fujiwara H, Yoshizaki T, Hosomochi K, Tajima A, Nakahara T, Kiyono T, Muramatsu M
Scientific reports 2018 Jun 27;8(1):9745
Scientific reports 2018 Jun 27;8(1):9745
Lead (Pb) induced ATM-dependent mitophagy via PINK1/Parkin pathway.
Gu X, Qi Y, Feng Z, Ma L, Gao K, Zhang Y
Toxicology letters 2018 Jul;291:92-100
Toxicology letters 2018 Jul;291:92-100
Aqueous extracts of Paeonia suffruticosa modulates mitochondrial proteostasis by reactive oxygen species-induced endoplasmic reticulum stress in pancreatic cancer cells.
Liu YH, Weng YP, Tsai HY, Chen CJ, Lee DY, Hsieh CL, Wu YC, Lin JY
Phytomedicine : international journal of phytotherapy and phytopharmacology 2018 Jul 15;46:184-192
Phytomedicine : international journal of phytotherapy and phytopharmacology 2018 Jul 15;46:184-192
miR-30a Remodels Subcutaneous Adipose Tissue Inflammation to Improve Insulin Sensitivity in Obesity.
Koh EH, Chernis N, Saha PK, Xiao L, Bader DA, Zhu B, Rajapakshe K, Hamilton MP, Liu X, Perera D, Chen X, York B, Trauner M, Coarfa C, Bajaj M, Moore DD, Deng T, McGuire SE, Hartig SM
Diabetes 2018 Dec;67(12):2541-2553
Diabetes 2018 Dec;67(12):2541-2553
PE_PGRS3 of Mycobacterium tuberculosis is specifically expressed at low phosphate concentration, and its arginine-rich C-terminal domain mediates adhesion and persistence in host tissues when expressed in Mycobacterium smegmatis.
De Maio F, Battah B, Palmieri V, Petrone L, Corrente F, Salustri A, Palucci I, Bellesi S, Papi M, Rubino S, Sali M, Goletti D, Sanguinetti M, Manganelli R, De Spirito M, Delogu G
Cellular microbiology 2018 Dec;20(12):e12952
Cellular microbiology 2018 Dec;20(12):e12952
Mitophagy inhibits proliferation by decreasing cyclooxygenase-2 (COX-2) in arsenic trioxide-treated HepG2 cells.
Niu Z, Zhang W, Gu X, Zhang X, Qi Y, Zhang Y
Environmental toxicology and pharmacology 2016 Jul;45:212-21
Environmental toxicology and pharmacology 2016 Jul;45:212-21
ATM mediates spermidine-induced mitophagy via PINK1 and Parkin regulation in human fibroblasts.
Qi Y, Qiu Q, Gu X, Tian Y, Zhang Y
Scientific reports 2016 Apr 19;6:24700
Scientific reports 2016 Apr 19;6:24700
Cadmium induces mitophagy through ROS-mediated PINK1/Parkin pathway.
Wei X, Qi Y, Zhang X, Qiu Q, Gu X, Tao C, Huang D, Zhang Y
Toxicology mechanisms and methods 2014 Oct;24(7):504-11
Toxicology mechanisms and methods 2014 Oct;24(7):504-11
Mutations in the SPG7 gene cause chronic progressive external ophthalmoplegia through disordered mitochondrial DNA maintenance.
Pfeffer G, Gorman GS, Griffin H, Kurzawa-Akanbi M, Blakely EL, Wilson I, Sitarz K, Moore D, Murphy JL, Alston CL, Pyle A, Coxhead J, Payne B, Gorrie GH, Longman C, Hadjivassiliou M, McConville J, Dick D, Imam I, Hilton D, Norwood F, Baker MR, Jaiser SR, Yu-Wai-Man P, Farrell M, McCarthy A, Lynch T, McFarland R, Schaefer AM, Turnbull DM, Horvath R, Taylor RW, Chinnery PF
Brain : a journal of neurology 2014 May;137(Pt 5):1323-36
Brain : a journal of neurology 2014 May;137(Pt 5):1323-36
Proteomic analysis of rhein-induced cyt: ER stress mediates cell death in breast cancer cells.
Huang HJ, Lin CC, Chou HC, Chen YW, Lin ST, Lin YC, Lin DY, Lyu KW, Chan HL
Molecular bioSystems 2014 Dec;10(12):3086-100
Molecular bioSystems 2014 Dec;10(12):3086-100
Hyaluronic acid-dependent protection against UVB-damaged human corneal cells.
Li JM, Chou HC, Wang SH, Wu CL, Chen YW, Lin ST, Chen YH, Chan HL
Environmental and molecular mutagenesis 2013 Jul;54(6):429-49
Environmental and molecular mutagenesis 2013 Jul;54(6):429-49
Quercetin-induced cardioprotection against doxorubicin cytotoxicity.
Chen JY, Hu RY, Chou HC
Journal of biomedical science 2013 Dec 20;20:95
Journal of biomedical science 2013 Dec 20;20:95
Basal and stress-induced Hsp70 are modulated by ataxin-3.
Reina CP, Nabet BY, Young PD, Pittman RN
Cell stress & chaperones 2012 Nov;17(6):729-42
Cell stress & chaperones 2012 Nov;17(6):729-42
Quantitative proteomic analyses of human cytomegalovirus-induced restructuring of endoplasmic reticulum-mitochondrial contacts at late times of infection.
Zhang A, Williamson CD, Wong DS, Bullough MD, Brown KJ, Hathout Y, Colberg-Poley AM
Molecular & cellular proteomics : MCP 2011 Oct;10(10):M111.009936
Molecular & cellular proteomics : MCP 2011 Oct;10(10):M111.009936
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Supportive validation
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- Experimental details
- HSP60 antibody detects HSPD1 protein by western blot analysis.A. 50 ?g rat brain lysate/extract7.5% SDS-PAGEHSP60 antibody (GTX110089) dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
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- Experimental details
- Sample (30£gg whole cell lysate)A:H1299B: HeLa S3(GTX14654)C:MOLT4 (GTX27912)7.5% SDS PAGEGTX110089 diluted at 1:10000
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- GeneTex (provider)
- Main image
- Experimental details
- Sample (20 ?g of whole cell lysate) A: mouse brain 7.5% SDS PAGE GTX110089 diluted at 1:20000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP60 antibody (GTX110089) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP60 antibody (GTX110089) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of methanol-fixed A431, using HSP60 (chaperonin)(GTX110089) antibody at 1:200 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HSP60 antibody detects HSP60 protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HSP60 protein stained by HSP60 antibody (GTX110089) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.Blue: Hoechst 33342 staining.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HSP60 antibody detects HSP60 protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: HSP60 protein stained by HSP60 antibody (GTX110089) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HSP60 antibody detects HSP60 protein at mitochondria on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. HSP60 antibody (GTX110089) dilution: 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HSP60 antibody detects HSP60 protein at mitochondrion on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. HSP60 antibody (GTX110089) dilution: 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HSP60 antibody detects HSP60 protein at mitochondrion on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. HSP60 antibody (GTX110089) dilution: 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HSP60 antibody detects HSP60 protein at mitochondria on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. HSP60 antibody (GTX110089) dilution: 1:500.
