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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- 681502 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#681502, RRID:AB_2566474
- Product name
- Purified anti-HSP60
- Antibody type
- Monoclonal
- Antigen
- Purified recombinant fragment (265-462 a.a.) of human HSP60 expressed in E. coli.
- Description
- HSP60, a mitochondrial chaperonin, is essential for the refolding, assembly, and transportation from the cytoplasm to mitochondrial matrix. It shares a high degree of homology and function similarity to bacteria GroEL. Studies also show that HSP60 may participate in some autoimmune diseases along with autosomal dominant and autosomal recessive neurodegenerative disorders.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- P83G8
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Total cell lysate from rat pancreas (lane 1), mouse spleen (lane 2), HepG2 (lane 3), HeLa (lane 4), A549 (lane 5), and IMR32 (lane 6) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified anti-HSP60 (clone P83G8) antibody. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection.
- Conjugate
- Unconjugated
