GTX22795

antibody from GeneTex

Targeting: HSPD1 GroEL, HSP60, SPG13

Western blot (Data presented on provider website)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

GTX22795

Provider

GeneTex

Proper citation

GeneTex Cat#GTX22795, RRID:AB_367065

Product name

HSP60 antibody [A57-B9]

Antibody type

Monoclonal

Reactivity

Bacteria

Host

Mouse

Storage

Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunofluorescent analysis of Heat Shock Protein 60 using Heat Shock Protein 60 Monoclonal Antibody (GTX22795) shows staining in BEAS-2B Cells. Heat Shock Protein 60 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Heat Shock Protein 60 (GTX22795) at a dilution of 1:100 over night at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunofluorescent analysis of Heat Shock Protein 60 using Heat Shock Protein 60 Monoclonal Antibody (GTX22795) shows staining in Hela Cells. Heat Shock Protein 60 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Heat Shock Protein 60 (GTX22795) at a dilution of 1:100 over night at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunofluorescent analysis of Heat Shock Protein 60 using Heat Shock Protein 60 Monoclonal Antibody (GTX22795) shows staining in 293Cells. Heat Shock Protein 60 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Heat Shock Protein 60 (GTX22795) at a dilution of 1:20 over night at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody.