AM03128PU-N

antibody from OriGene

Targeting: HSPD1 GroEL, HSP60, SPG13

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

AM03128PU-N

Provider

OriGene

Product name

Hsp60 (HSPD1) (1-573) mouse monoclonal antibody, clone 2E4, Purified

Antibody type

Monoclonal

Description

Hsp60 (HSPD1) (1-573) mouse monoclonal antibody, clone 2E4, Purified

Host

Mouse

Conjugate

Unconjugated

Epitope

HSPD1

Isotype

IgG

Antibody clone number

2E4

Vial size

100 µl

Concentration

1.0 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis : Cell lysates of Hela (30 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human HSP60 No. (1/1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemistry : Paraffin embedded sections of human colon cancer tissue were incubated with anti-human Hsp60No. (1/50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).

Validation comment

IHC