AM03145PU-N

antibody from OriGene

Targeting: HSPD1 GroEL, HSP60, SPG13

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AM03145PU-N

Provider

OriGene

Product name

Mouse Monoclonal Anti-HSPD1 Antibody, Purified

Antibody type

Monoclonal

Description

Mouse Monoclonal Anti-HSPD1 Antibody, Purified

Reactivity

Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Guinea Pig, Hamster, Porcine, Rabbit, Simian, Yeast

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Antibody clone number

Clone LK2

Vial size

50ug

Concentration

1.0 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Gels were loaded with 1, 2.5, 5.0 and 7.5 ug/lane of HeLa and Raw204.4 cell lysates. 0.2 ug/ml primary antibody was used, blocking buffer (Blotto; 5% dry milk powder in TBST). R1253HRP anti Mouse IgG (H+L) secondary antibody at 1/20.000 dilution was used.

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemistry analysis of Listeria infected mice spleens 6 days after infection with L-monocytogenes using? (Clone LK2).

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Hsp60 visualized using (Clone LK2), tested on Human colon cancer cells.

Validation comment

IHC