AM12011PU-N

antibody from OriGene

Targeting: HSPD1 GroEL, HSP60, SPG13

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AM12011PU-N

Provider

OriGene

Product name

Mouse Monoclonal Anti-HSPD1 Antibody, Purified

Antibody type

Monoclonal

Description

Mouse Monoclonal Anti-HSPD1 Antibody, Purified

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Antibody clone number

Clone LK-1

Vial size

50ug

Concentration

1.0 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Gels were loaded with 1, 2.5, 5.0 and 7.5 ug/lane of HeLa and Raw204.4 cell lysates. 0.2 ug/ml primary antibody was used, blocking buffer (Blotto; 5% dry milk powder in TBST). R1253HRP anti Mouse IgG (H+L) secondary antibody at 1/20.000 dilution was used.

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemistry of human skin fibroblasts (Left: control, Right: 24 hours after 7th passage of senescence).

Validation comment

IHC