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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
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- Product number
- MAB1121 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB1121, RRID:AB_1237995
- Product name
- HSPD1 monoclonal antibody, clone 2E4
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against full length recombinant HSPD1.
- Isotype
- IgG
- Antibody clone number
- 2E4
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Hsp60 regulation of tumor cell apoptosis.
The mitochondrial chaperonin hsp60 is required for its own assembly.
Ghosh JC, Dohi T, Kang BH, Altieri DC
The Journal of biological chemistry 2008 Feb 22;283(8):5188-94
The Journal of biological chemistry 2008 Feb 22;283(8):5188-94
The mitochondrial chaperonin hsp60 is required for its own assembly.
Cheng MY, Hartl FU, Horwich AL
Nature 1990 Nov 29;348(6300):455-8
Nature 1990 Nov 29;348(6300):455-8
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Supportive validation
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- Abnova Corporation (provider)
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- Experimental details
- Western blot analysis of cell lysates of HeLa (30 ug) were resolved by SDS - PAGE , transferred to NC membrane and probed with HSPD1 monoclonal antibody , clone 2E4 (1 : 1000) (Cat # MAB1121). Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and an ECL detection system.
